According to the product description....
"..extremely short PCR protocols..proofreading DNA polymerase...error rate when using Phusion Flash PCR Master Mix is 9.5 x 10-7 ..."
Ready-to-use master mix minimizes required pipetting steps: Only template and primers need to be added by the user. In addition, the unique composition of the master mix enables the use of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less.
Yeah, it really reduces my pipetting time, pcr time (short cycles), and with 15 secs I can get my desired products but have some non-specific bindings as well. Might as well reduce the time.
Ready-to-use master mix minimizes required pipetting steps: Only template and primers need to be added by the user. In addition, the unique composition of the master mix enables the use of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less.
Yeah, it really reduces my pipetting time, pcr time (short cycles), and with 15 secs I can get my desired products but have some non-specific bindings as well. Might as well reduce the time.
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